For the first time, an integrated PCR and sequence molecule analysis has been performed. Heritable recessive point mutations of two different genes of Drosophila melanogaster induced by 60Co γ-ray at the dose of 40 Gy were examined. The results from the PCR analysis of the above-mentioned gene mutations were presented at the seminar, demonstrating different frequency of two main types of DNA changes (PCR- and PCR+).

A prediction of hereditary effects in the offspring of irradiated parents remains one of the most important tasks and at the same time a fundamental issue for radiogenetics of generative cells for over 60 years. Nowadays, the relevance of this problem increases even more, in view of expanding human exposure to ionizing radiation on the Earth and in space, as well as the facing potential technical or military nuclear accidents.

 

 

Results of the molecular (PCR) analysis of γ- and neutron-induced mutations of the white (w) gene localized in the Хchromosome of Drosophila melanogaster are reported (authors: E.V. Kravchenko, A.N. Russakovich, F. Elnoamani, S.V. Dubovik, S.E. Hassab-El-Nabi,, M.V. Aleksandrova, and I.D. Aleksandrov). Dose dependences of gene mutations induces by γrays and neutrons were preliminarily studied and found to be linear.
 

Genetic effect of ionizing radiation on generative cells (gametes) is characterized not only by a diversity of observed changes in individual genes and the genome as a whole but also by the danger that these changes constitute for the coming generations.


The observed diversity stems from both the features of the DNA organization of the nanotarget (gene) and the microtarget (genome) and the character of the ionizing particle energy distribution in these targets. The topics covered at the seminar were modern concepts of the gene and genome DNA organization, energy distribution in the genome (within the modern track structure theory), and the spectrum of primary (theoretically expected0 and observed (from the sequence analysis of radiation-induced mutations) inherited DNA changes.

For more detail see here

 

At DLNP, multidisciplinary investigations of genetic laws at the molecular level are being carried out using up-to-date instruments for the whole genome analysis of DNA and unique particle and radiation sources. 

These investigations allow one to understand what kind of and how many mutations arise in DNA under the effect of radiation and what kind of radiation induces the largest changes in DNA. Mutations produced by ionizing radiation provide a lot of research material for studying the functioning of various genes and their regulation mechanisms, search for new regulation elements, and development of genetic-engineering structures with controlled gene functioning.  

A possibility of working with both individual genes and whole genomes allows interesting experimental research to be conducted at JINR in the field of molecular genetics and radiobiology for studying the structure and functions of genes and the inherited changes in DNA resulting from the exposure to ionizing radiation.

Scientific consultant: E.V. Kravchenko (Candidate of Sciences, Biology).

3D animation, setting, narration: S. Gurskii.